Recombinant production of proteins is one of the great promises of the biotechnology industry. A high level of protein expression and optimal purification is imperative for protein biology research groups in academia as well as industry. However producing these novel proteins is not a simple task and can be very challenging because so many factors may influence the process.
It is a dynamic field that is constantly evolving with the emergence of new hosts, new genetic elements, enhancement of productivity by strain engineering, systems-wide investigation of the host physiology, optimization of the culture conditions, development of efficient purification strategies and high throughput in vitro refolding screening etc. to meet the spectrum of research, development and commercial needs.
Depending on specific applications, the system chosen for protein expression can be critical for the end point use of the desired protein.
Although certain trends are apparent, there is no one-size-fits-all approach for successful production of recombinant proteins. Considerations of cell type, plasmid construction, and purification strategy are all part of the process of developing a successful production strategy of recombinant protein.
All the expression systems available have their merits and demerits concerning yield, proper folding, post-translational modification (PTM), cost, speed and ease of use.
We carry out research to understand the molecular background and physiology of protein production tailored to the customer’s requirements. We ensure that our services and products meet customer’s needs genuinely.